STEVOR proteins interact with components of the ankyrin complex. (A) Schematic representation of STEVOR recombinant protein overexpressed in the Full-myc line. Black bars represent recombinant his-tagged Stevor-Cter and N-terminal (Stevor-Nter) domains. (B) Immunoprecipitation of stage III V GIE lysates from the Full-myc and the Null lines with anti-myc and anti-GST antibodies. Immunoblot was probed with a rabbit polyclonal antibody directed against STEVOR (PFL2610w). (C) MS analysis of immunoprecipitates from the Full-myc and the Null line with anti-myc and anti-GST antibodies. *Average number of peptides for 4 independent experiments. (D) Pulldown assays of recombinant spectrin dimer (Sigma-Aldrich) (top and middle panel) or recombinant Band 3 (bottom panel) with recombinant his-tagged STEVOR C-terminal (Stevor-Cter) or N-terminal (Stevor-Nter) domains on nickel beads. Input represents 10% of recombinant proteins used for pulldowns. Immunoblot were probed with a rabbit anti-spectrin α antibody (top panel), a rabbit anti-spectrin β antibody (middle panel) or a rabbit anti-band 3 antibody (bottom panel).