Pretreatment of NK effector cells with DAC or 5-azacytidine did not compromise BI 836858–mediated ADCC or NK-cell activation against AML blasts. NK cells from patients were exposed to DAC (500 nM) or 5-azacytidine (2 µM) for 48 hours before incubation with AML blasts coated with BI 836858 or BI 836847 for the ADCC assay (10 μg/mL, E:T = 3:1; A) or CD107a assay (5 μg/mL, E:T = 2:1; B). Shown are representative data of autologous NK cells and AML primary blasts from 2 patients. **P < .01, ***P < .001. ns, not significant.