BI 836858 elicits potent NK-cell–mediated ADCC effect as well as NK-cell activation against primary AML blasts. (A-B) AML primary blasts were precoated with different concentrations of BI 836858, and then cocultured with NK cells from healthy donors at an E:T of 3:1 for the ADCC assays. The Fc-engineered, nonbinding BI 47 antibody was used as negative control antibody at the same E:T for comparison. Representative cytotoxicity for 1 blast donor (A) and summary fold changes for 8 different donors (B) data are shown. (C-D) AML primary blasts were precoated with 5 μg/mL BI 836858 or BI 836847, and then cocultured with NK cells at an E:T of 2:1 for the CD107a assays. Representative CD107a expression for 2 donors (C) and summary fold changes for 8 different donors (D) are shown. *P < .0001, **P < .01, ***P < .001. ns, not significant.