Correlation between MUC1 and MYC expression in primary MM cells. (A-B) Primary CD138⁺ MM cells obtained from patient 1 (A) and patient 2 (B) were stained for MUC1-C (green) and MYC (red) expression. DAPI counterstain was used to visualize nuclei. Immunofluorescence images were obtained using a Nikon Ti inverted microscope at a magnification of ×20. (C) Microarray data from Oncomine database are expressed as box plots (25th-75th percentiles) for MYC (top panels) and MUC1 (bottom panels) expression in NPCs (n = 5), MGUS (n = 11), MM (n = 133), and PCL (n = 9). The data were log₂ transformed and median centered (dark lines). *P value of <.05. (D-E) Microarray gene expression data from GEO data sets GSE2658 (D; n = 559) and GSE26760 (E; n = 304) was robust multiarray average normalized and the correlation between MUC1 and MYC expression in MM patients was assessed by the Spearman rank-order correlation, where P < .05 was considered as statistically significant. n.s., not significant.