Hepatocellular necrosis and fibrin(ogen) deposition in livers of ANIT-exposed Fibγ^390-396A and FXIII^−/− mice. WT, Fibγ^390-396A, and FXIII^−/− mice were fed control a diet (AIN-93M) or an identical diet containing 0.025% ANIT for 4 weeks. Representative photomicrographs show liver sections stained for H&E or fibrin(ogen) (brown) from: (A) Fibγ^390-396A mice, (C) FXIII^−/− mice, and (A,C) corresponding WT mice exposed to ANIT. Arrows indicate areas of bile duct hyperplasia. Arrowheads indicate fibrin(ogen) deposits within areas of hepatocellular necrosis. Asterisks indicate focal hepatocellular necrosis or bile infarcts. Images are ×200 except hematoxylin and eosin (H&E) stained sections in (C), which are ×100. (B,D) Area of necrosis was determined as described in “Methods.” (E) Representative photomicrographs show liver sections stained for sirius red (×200). Arrows indicate peribiliary collagen deposition. Asterisks indicate focal hepatocellular necrosis (ie, bile infarcts). (F) Sirius red staining was quantified as described in “Methods.” Data are expressed as mean + SEM; n = 4 to 16 mice per group.*P < .05 vs ANIT-exposed WT mice.