Syk and PLCγ2 are involved in the signaling pathway in platelets induced by CAP-PEs. (A-G) Gel-filtered human platelets were (A-B,G) preincubated with 3 μM Syk inhibitor BAY61-3606 (BAY61) or (C,F) Src kinase family inhibitor PP2 (10 μM) or (D-E) PLC inhibitor U73122 (10 μM), followed by CAP-PEs (CHP-PE or CEP-PE) stimulation. P-selectin expression was assessed by FACS analysis (A,D), Syk phosphorylation (B-C), and PLCγ2 phosphorylation (E-G) were determined by western blot analysis using phospho-specific antibodies of the respective proteins. (H) Murine platelets isolated by gel filtration from WT; TLR2^−/− and Lyn^−/− mice were stimulated with CAP-PEs (CEP-PE) for 5 minutes. PLCγ2 phosphorylation was determined by western blot analysis using phospho-specific antibody.