CD40LG-edited T cells represent a diverse TCR repertoire and are stable in vivo. (A) Ratio of the percentage of GFP expression pretransplant: 5 weeks post-NSG transplant. HC 50/50 Mix, mice transplanted with 50% mock-edited and 50% GFP⁺ sorted CD40LG[GFP.CD40L.WPRE]-edited healthy (HC) T cells; HC Edited and X-HIGM Edited, mice transplanted with bulk CD40LG[GFP.CD40L.WPRE]-edited T cells from a healthy control (input cells 52% GFP⁺) or an X-HIGM patient (input cells 19% GFP⁺), respectively. Bars show mean ± SEM. (B) CD40L surface expression in resting and P/I-activated human T cells recovered 5 weeks posttransplantation. (C) TCRVB CDR3 spectratyping analysis of CD4⁺ T cells prior to transplant. N = 3 (HC) and 1 (X-HIGM). Bars show the average and SEM of CDR3 subfamily complexity.