Figure 2
![Figure 2. CD40LG gene replacement with a functional CD40LG transgene. (A) CD40LG cDNA gene replacement templates targeting the 5′UTR region of exon 1 of CD40LG: CD40LG[GFP.CD40L.3′UTR] (top) and CD40LG[GFP.CD40L.WPRE] (bottom). Total AAV packaging sizes are 4616 and 4234 bp, respectively. (B) Average HDR rate (percentage GFP+; n = 4 separate healthy male donors) of each gene-editing template in CD4+ T cells. (C) GFP (x-axis) and CD40L (y-axis) coexpression or (D) CD40-muIg binding in T cells edited with cDNA gene replacement templates. (Top row) Resting; (bottom) P/I activated. *Plots in panels C and D were generated at separate times, using different donor T cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/127/21/10.1182_blood-2015-11-683235/4/2513f2.jpeg?Expires=1722710209&Signature=FYzB4QHuhjJa1wDeHRvSFdfUJwC2reaZaLhDbPOIcaUt5b3T6jiPXkWUNui-ciWerBXMTEWF0jGuUbwyPRU~8x-fao3o2qqVWbOxybf30vQlw~A-S~d2~bF1a77tflJvPREo7VmD1GlubAr~tGyyT7iL0bRFV92XEO1jFkK2QZYqszwDDf7eJOrywdmpb5skjqeKLzZwd16vmmtV5RPf49V~NpaY~tY-Qt8yCFOC1VK2IBKJEgQxs9CirqtX3pdnoZELDjwWzUBK7Far6-RiTipLEet2xF4vM2Wt677OuWAf5Al4PiZpsvSkEOK1-piqVYvYzQMC8ZXw1oAz6t0rLA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD40LG gene replacement with a functional CD40LG transgene. (A) CD40LG cDNA gene replacement templates targeting the 5′UTR region of exon 1 of CD40LG: CD40LG[GFP.CD40L.3′UTR] (top) and CD40LG[GFP.CD40L.WPRE] (bottom). Total AAV packaging sizes are 4616 and 4234 bp, respectively. (B) Average HDR rate (percentage GFP+; n = 4 separate healthy male donors) of each gene-editing template in CD4+ T cells. (C) GFP (x-axis) and CD40L (y-axis) coexpression or (D) CD40-muIg binding in T cells edited with cDNA gene replacement templates. (Top row) Resting; (bottom) P/I activated. *Plots in panels C and D were generated at separate times, using different donor T cells.
