CCR5 mutations induced in CD34⁺ HSPCs ex vivo are detected in multiple post-transplant time points in vivo. Total gDNA was collected from ex vivo cultured CD34⁺ HSPCs from animal IDs A11217 (A), A11210 (B), Z12161 (C), and Z12220 (D), 5 to 6 days following electroporation (“infusion product”), or from total leukocytes collected from each animal 6 to 12 and 189 to 198 days following transplantation of these cells. CCR5 disruption was measured from total gDNA by Illumina MiSeq and the presence of the indicated mutants was tracked over time in each animal. The 50 most frequent mutants that were detected in more than one time point in a given animal (“recurrent mutants”) are shown in colored bars. Frequency of each mutant from the indicated animal/time point is displayed as a proportion of the total pool of mutants that were detected. In each graph, the top bars (dark gray) represent the sum of mutants that were detected in only one time point (“non-recurrent mutants”), and the second bars (teal) represent the sum of recurrent mutants that were not among the 50 most frequent. Single base pair point mutations were excluded from the analysis and infusion product from A11210 was not available for analysis. ND, not determined.