American Society of Hematology

Figure 1

$Figure 1. Schematic of electroporation-based autologous transplant protocol. Primed bone marrow (BM) aspirates were collected from 4 pigtailed macaques, and CD34+ cells were isolated as described previously. Following overnight ex vivo culture, cells were electroporated in the presence of mRNA encoding CCR5-targeting ZFNs, and incubated overnight at 30°C. The next day, cells were collected, pulsed with prostaglandin E2, and infused into the autologous animal. During the 48-hour ex vivo culture period, the animal received a fractionated dose of 1020 cGy TBI. FLT-3, FMS-like tyrosine kinase 3; SCF, stem cell factor; TPO, thrombopoietin.$

Schematic of electroporation-based autologous transplant protocol. Primed bone marrow (BM) aspirates were collected from 4 pigtailed macaques, and CD34⁺ cells were isolated as described previously. Following overnight ex vivo culture, cells were electroporated in the presence of mRNA encoding CCR5-targeting ZFNs, and incubated overnight at 30°C. The next day, cells were collected, pulsed with prostaglandin E2, and infused into the autologous animal. During the 48-hour ex vivo culture period, the animal received a fractionated dose of 1020 cGy TBI. FLT-3, FMS-like tyrosine kinase 3; SCF, stem cell factor; TPO, thrombopoietin.

This Feature Is Available To Subscribers Only