Antibrain and anti-CL antibodies were detected in FPI mice. (A) Immunoreactivity to MBH of 1/1000 diluted plasma from FPI mice at different postinjury times (n = 6/point; repeated measures ANOVA, *P < .01 vs baseline before FPI). (B) Binding of IgGs purified from sham or FPI mice to MBH (n = 6/dose; paired Student t test, *P < .01). (C) Anti-brain IgGs (0.5 μg/mL) from FPI mice were incubated with increasing concentrations of CL for 30 minutes, and then with MBH for 30 minutes at RT (n = 5-7/dose; repeated measures ANOVA, *P < .01 vs IgG at 0 CL). (D, top) CL blotted to nitrocellulose membrane was probed with IgGs (0.25 μg/mL) from sham and FPI mice (10 days post-FPI) in the presence of increasing amounts of MBH. (Bottom) Phosphatidylcholine (PC), PS, and CL blotted onto nitrocellulose membrane (18 μg/spot) were probed with the CL antibody purified from plasma collected 10-14 days after FPI from TBI mice.