Correlation of VWF content with thrombus composition, stroke etiology, and treatment. VWF, red blood cell, and fibrin positive area were quantitatively analyzed for each of the thrombi. (A) A strong negative linear association was found between red blood cell content and VWF positive area. R² = 0.4248; P < .0001. (B) A positive linear association was found between fibrin content and VWF positive area. R² = 0.1867; P = .0085. (C) No significant difference in VWF staining was observed with different types of stroke etiology. (D) Thrombolysis did not affect thrombus VWF content (P = .9172). CE, cardioembolic; LAA, large artery atherosclerosis.