Bmp6^−/−-Tmprss6^−/− mice are neither anemic nor iron overloaded. F2 littermate mice (CD1-C57BL/6 mixed background) of the different genotypes (5 to 9 mice per group) were analyzed for (A) serum EPO level; (B) liver non-heme iron content; (C) liver Hamp mRNA expression; and (D) liver pSmad-5 relative to total Smad5 protein. Results are expressed as mean ± SD and compared by ANOVA followed by Sidak’s multiple comparison tests. ****P < .0001; **P < .01; *P < .05. Point estimates of the fold changes in gene expression (2^-ΔΔCt) are shown on the graphs.