Representative examples of clonal evolution observed in a 48-sample longitudinal analysis. Illustration of 4 representative CLL cases of clonal evolution showing the decrease or expansion of the BIRC3-, TP53-, SF3B1-, ATM-, or NOTCH1-mutated clone. Time 0 corresponds to the diagnosis time point. Each circle represents a unique mutation and its size is proportional to the VAF of the mutation corrected by the sample’s tumor purity. Each mutation is represented at the time point at which a tumor sample was collected. B+O, bendamustine, ofatumumab; B+R, bendamustine, rituximab; Chl, chlorambucil; FC, fludarabine, cyclophosphamide; L, lenalidomide; RFCM, rituximab, fludarabine, cyclophosphamide, mitoxantrone.