Molecular profile and schematic diagram of clonal and subclonal TP53, ATM, BIRC3, SF3B1, and NOTCH1 mutations. (A) VAF of the mutations identified by NGS in each of the studied genes. Blue bars correspond to clonal mutations (VAF ≥12%) whereas orange bars to the subclonal mutations (VAF <12%). (B) Schematic diagram of TP53, ATM, BIRC3, SF3B1, and NOTCH1. Exons are represented by boxes and the main protein domains are colored. Color-coded shapes indicate the position and type of the mutation. Variants represented on the top of the protein correspond to high-frequency mutations (clonal) whereas variants represented under the diagram correspond to low-frequency mutations (subclonal). Shaded area corresponds to the region sequenced. (C) Comparison of the molecular profile of the identified clonal and subclonal mutations. Each pair of bars represent clonal (dark) and subclonal (light) mutations. No statistical differences were observed by the Fisher exact test.