American Society of Hematology

Figure 4

$Figure 4. GPR56 is a stable LSC marker in culture conditions. (A) Experimental setup of in vitro experiments to test stability of GPR56 in culture conditions. (B) Percentage of GPR56+ cells after 7 days in vitro in optimized culture condition (SR1+UM729) or vehicle DMSO compared with uncultured cells (given as ratios day 7/day 0, P < .0001, Wilcoxon matched pairs signed-rank test). (C) Representative FACS profile of sample 11H160 cultured for 7 days in optimized culture condition (SR1+UM729) or vehicle DMSO. Compare Figure 3D, left panel, for day 0 profile. (D) Human engraftment levels in recipient mice (horizontal bars indicate means) generated by sorted fractions after 14 weeks in NSG mice (lower panel) shown for 3 samples after 4 days in culture with SR1 and UM729.$

GPR56 is a stable LSC marker in culture conditions. (A) Experimental setup of in vitro experiments to test stability of GPR56 in culture conditions. (B) Percentage of GPR56⁺ cells after 7 days in vitro in optimized culture condition (SR1+UM729) or vehicle DMSO compared with uncultured cells (given as ratios day 7/day 0, P < .0001, Wilcoxon matched pairs signed-rank test). (C) Representative FACS profile of sample 11H160 cultured for 7 days in optimized culture condition (SR1+UM729) or vehicle DMSO. Compare Figure 3D, left panel, for day 0 profile. (D) Human engraftment levels in recipient mice (horizontal bars indicate means) generated by sorted fractions after 14 weeks in NSG mice (lower panel) shown for 3 samples after 4 days in culture with SR1 and UM729.

This Feature Is Available To Subscribers Only