Significantly different phenotype in mice with Pten deletion induced at 6 weeks after birth in comparison with mice induced at PND8. (A) Photographs of organs from representative littermate mice with Pten deletion induced at PND8 (euthanized at age 3 weeks) or at age 6 weeks (euthanized at age 9 weeks). Hepatosplenomegaly and significant enlargement of lymph nodes and thymus are demonstrated in mice with PtenΔ/Δ; Nf1^+/− induced at age 6 weeks. (B) Micrographs demonstrate BM with increased lymphoblasts and associated lymphocyte infiltration in the lung and liver (indicated by arrows), spleen, and thymus in mice with PtenΔ/Δ; Nf1^+/−. BM cytospins were stained by MGG; lung with HE; liver, spleen, and thymus with HE and IHC with antibody against CD3. Images were acquired by an Aperio CS2 scanner (additional relevant FACS data are presented in supplemental Figure 6).