Analysis of progenitor cells from the BM and spleens of PtenΔ/Δ; Nf1^+/− mice induced at PND8 and of control littermates at age 3 weeks (2 weeks post–poly[I:C]). Colony-forming unit–granulocyte-macrophage (CFU-GM) assays: after serum starvation for 4 hours, 5 × 10⁴ BM cells (A-C) or 2 × 10⁵ spleen cells (D-F) were seeded in triplicate on 1-mL semisolid cultures in 35-mm plates with 0.3% agar and McCoy’s 5A Medium supplemented with nutrients and 15% fetal calf serum containing various concentrations of recombinant mouse GM-CSF or IL-3. (A-B,D-E) Data show there was no significant aberrant curve shift for GM-CSF and IL-3 sensitivities. (C,F) FACS analysis suggests hematopoietic migration from the BM to the spleen in mice with PtenΔ/Δ; Nf1^+/−. LIN⁻, lineage markers negative; HPC, LIN⁻Scal-1⁻c-Kit⁺; LSK, LIN⁻Scal-1⁺c-Kit⁺. Data are presented as mean ± SD. *P < .05; **P < .01; ***P < .001. Additional supportive data are presented in supplemental Figure 3.