Defective clearance of polyreactive B cells in germinal centers. The proportion of polyreactive (black bar) and nonpolyreactive (white bar) single-cell EBV immortalized B-cell clones from sorted B-cell subpopulations in (A) 2 HD and (B) P1. The numbers above the bars indicate numbers of clones analyzed. Polyreactivity was defined as positive ELISA titer in the same clone to ≥3 antigens. ***P < .001 (Fisher’s exact test).