Analysis of Dnmt3b-regulated genes in human AML data sets. (A-B) Candidate genes were analyzed for their prognostic impact in human leukemia using published patient data and the Leukemia Gene Atlas. Gene expression data were grouped into quartiles, and survival outcome was analyzed in a patient data set published in 2009 by Verhaak et al.64 Shown is overall survival in AML patients for low (0% to 25%), intermediate (25% to 75%), and high (75% to 100%) expression levels of the candidate genes GPR56 (P < .001) (A) and H1F0 (P = .023) (B). (C) DNA methylation (DNAm) levels of candidate genes were analyzed in 194 AML patients using Illumina bead array data available from the TCGA database. Each column represents 1 patient, and methylation levels are color coded with blue representing low methylation and yellow representing high methylation. (D) Correlation between DNA methylation levels and gene expression for the identified target genes from AML patients in the TCGA database. Depicted is the correlation coefficient for all candidate genes. Overall, 34 genes showed significant negative correlation between methylation and expression.
