Dnmt3b expression increases the number of multipotent progenitor cells in nonleukemic mice. (A) Induction of Dnmt3b expression in nonleukemic Dnmt3b-knock-in mice increased the numbers of multipotent progenitor cells after 4 weeks (n = 3 per group). LT-HSC gated for LSK CD34⁻Flt3⁻; ST-HSC gated for LSK CD34⁺Flt3⁻; LMPP gated for LSK CD34⁺Flt3⁺. (B) White blood cell counts of wild-type (black circles) and Dnmt3b-knock-in (red circles) mice after 16, 28, and 44 weeks of in vivo induction of Dnmt3b expression. (C) Total cell counts for lymphocytes in peripheral blood of wild-type and Dnmt3b-knock-in mice at the indicated time points after induction of Dnmt3b. Each dot represents 1 individual mouse. (D) Survival of wild-type and Dnmt3b-knock-in mice after induction of Dnmt3b expression for up to 1 year (n = 6 for wild-type [WT] and n = 8 for Dnmt3b-knock-in mice). (E) Percentage of CD45.2⁺ donor-derived peripheral blood cells in primary recipients for up to 24 weeks posttransplantation. Transplanted cell doses are 10⁶ and 10⁵ cells mixed with 10⁵ competitor cells (n = 4 mice each). (F) Dnmt3b-knock-in total bone marrow cells were impaired in growth upon culture in the presence of IL-3, IL-6, and murine stem cell factor; n = 3 per group. Data in panels A-C and E-F are mean ± SD values. *P < .05; **P < .01; ***P < .001.