Platelet α_IIb and β₃ mRNA associates with Ago2. Pooled lysates (5 to 7 mice per group) from Dicer1^Pf4Δ/Pf4Δ or Dicer1^fl/fl mouse platelets were immunoprecipitated with an antibody against Ago2 or against the isotype control FLAG followed by immunoblotting (IB) for the indicated protein. (A) Total Ago2 protein expression in platelets from Dicer1^Pf4Δ/Pf4Δ or Dicer1^fl/fl mice. Blot shown is representative of 2 independent experiments. (B) Immunoprecipitated Ago2 protein expression in platelets from Dicer1^Pf4Δ/Pf4Δ or Dicer1^fl/fl mice. n = 1 platelet pools (pooled from 5 mice per group). (C) Immunoprecipitated RNA was extracted from IP, and α_IIb or β₃ mRNA association to Ago2 was estimated by quantitative real-time PCR (in duplicates). The results are normalized with Gapdh mRNA to calculate a fold change vs FLAG IP. Results are representative of 2 independent experiments demonstrating enrichment of α_IIb or β₃ mRNA in Ago2 IP in pools (pooled from 5 to 7 mice per group) of Dicer1^fl/fl platelet lysates, and n = 1 platelet pools per group (pooled from 5 mice per group) for the comparison with Dicer1^Pf4Δ/Pf4Δ animals.