HERC4 decreases MM cell proliferation and delays MM tumor growth in nude mice. (A) RPMI 8226 and MM1.S cells were infected with increased pLVX-HERC4 lentivirus. Cells were subjected to IB for the expression of HERC4 and c-Maf. (B) RPMI 8226 and MM1.S cells were infected with HERC4 lentivirus followed by MTT assay for cell viability. (C) RPMI 8226 cells infected with HERC4 or mock lentivirus were injected into SCID mice subcutaneously. Tumor weights were then measured at the end of the experiment. (D) Tumor samples from the myeloma xenografts were subjected to IB analysis for c-Maf, HERC4, and cyclin D2 with specific antibodies.