Relationship between VWF A2 domain thermostability and ADAMTS13 binding. (A) The competition binding percentage of the VWF A2 domain variants at saturating concentrations (500 nM) to ADAMTS13 is displayed. Results are means ± SEM of at least 3 independent experiments (the N1515Q/N1574Q/Y1544D variant is labeled as Y1544D/NQ/NQ). (B) The calculated mean T_m was plotted against the mean solution binding of 500 nM VWF A2 to 30nM iADAMTS13 for the variant and condition matched VWF A2 domain variants. Example data points are indicated on the graph, and the Spearman coefficient was calculated using linear regression (GraphPad).