The antioxidant effect of DF on ECs is caused by its interaction with the cell membrane. (A) Flow cytometry experiments reveal that DF has an antioxidant effect in front of an oxidative stimuli (H₂O₂), even in the presence of Wortmannin (W). Black bars correspond to intracellular ROS in SK cells induced by incubation with 1 μM H₂O₂, expressed as percentage of positive cells. White bars correspond to percentage of dead cells after exposing SK to a high concentration of H₂O₂ (50 μM). (B) The immunoblot image shows changes in the presence of the protein eNOS3 in SK exposed to 1 μM H₂O₂, without and with DF in the media and in the absence and presence of Wortmannin (W), as indicated. The bar diagram represents relative presence of eNOS3 vs control. Data corresponds to n = 4, being *P < .05 vs control and ^#P < .05 vs H₂O₂.