Gata2 is expressed by Venus⁻ cells after culture. Schematic diagram showing method and FACS analysis by which Gata2 expression was found in the progeny of sorted Venus⁻ HPCs. G2V YS tissue was FACS sorted into Venus⁻ and Venus⁺ fractions. Cells were subsequently seeded in methylcellulose, and colonies were analyzed after 10 days of culture. Colonies were harvested from the dish, cells were washed and stained (with anti-Gr1 and anti-Mac1 antibodies), and Venus, Gr1, and Mac1 expression was analyzed by FACS. FACS plots (top) indicate Venus expression in cells harvested from Venus⁻ (left) and Venus⁺ (right) CFU-C experiments. Note that both FACS analyses indicate Venus expression in both cultures. FACS plots (bottom) show Gr1 and Mac1 expression in Venus⁻ and Venus⁺ populations in both cultures and that cells harvested from the Venus⁺ culture show a more immature phenotype.