Localization of Gata2Venus-expressing cells in embryonic hematopoietic sites. Confocal images of a whole mount immunostained E10.5 Gata2Venus embryo showing (A) Venus (green), (B) CD31 (magenta), and (C) merged expression. Venus-expressing cells are detected in the AGM along the wall of the dorsal aorta (dotted lines), the FL, NT, and OB. (D) Confocal image of a transverse section through the E10.5 AGM. DAPI staining (blue), CD31 (red), and Venus fluorescence (green) revealed Gata2-expressing aortic endothelial and hematopoietic cluster cells and UG and FL cells. Enlarged images of D showing Gata2-expressing cells in (E) AGM (DA, dorsal aorta; UG, urogenital ridges; arrowheads indicate hematopoietic cluster) and (F) FL. Venus (green) and CD34 (red) fluorescence showing endothelial and hematopoietic cluster cells in (G) E9 paired aorta, (H) umbilical artery (UA) at E9, and (I) E11 aorta. Arrowheads indicate hematopoietic cluster. (J-M) Images of E10.5 YS section showing DAPI merged, Venus, CD31, and merged fluorescence. Arrow denotes an endothelial cell expressing Venus and CD31.