Proteomic analysis reveals that MARCKS is upregulated in proplatelet-producing MKS. Representative labeled spot maps of murine megakaryocytes cultured for 24 hours ± puromycin (250 μg/mL, final). (A) Gel showing 1.5× or greater differences in spot volume ratio. Blue = increase in Cy5/Cy3 (Puromycin/Untreated), red = increase in Cy3/Cy5 (Untreated/Puromycin). (B) All picked spots to date; of these, the 7 spots with the highest fold difference were subject to MS/MS analysis.