AKT activity is neither affected by FOXP1/S1PR2 signaling nor required for DLBCL cell survival. (A-B) AKT activity was assessed by phospho-S473–specific western blotting of the indicated cell lines 72 hours after transfection with FOXP1-specific or control siRNAs (A) or after transfection with the indicated expression plasmids (B). Total AKT and α-TUBULIN are shown as loading controls. (C) Cell viability of U-2932 cells was measured by CellTiter Blue assay 48 hours after transfection with expression plasmids encoding S1PR2 and a myristoylated, constitutively active form of AKT. Data were normalized to empty plasmid (pTCN) and represent means + SEM of 3 independent experiments. (D) Cell viability of the indicated cell lines was measured by CellTiter Blue assay after 24 hours of treatment with the indicated concentrations of the AKT inhibitor MK-2206 or the vehicle control (dimethylsulfoxide). Data were normalized to vehicle control (dimethylsulfoxide) and are represented as means + SEM of 3 independent experiments.