PI3K-C2α is a key regulator of plasma membrane/skeleton integrity. (A) Cholesterol, phospholipids, and fatty acids from resting platelets were analyzed as described in Methods. MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; SAFA, saturated fatty acids. (B) Western blot analysis of actin, spectrin, myosin, vinculin, filamin, moesin, GPIIb, and GPIbα from isolated membrane skeleton (Mbr skeleton) and whole lysates (WCL) of resting platelets (mean ± SEM; n = 4–7; *P < .05, **P < .01 vs WT according 1 sample t test).