Deregulated NF-κB activity in IκBα^−/−IκBε^−/−cRel^−/−TNF^−/− leads to reduction in T1 cells and increased MZ B-cell generation. FACS analysis of peripheral B-cell development in whole splenic extracts from wild-type, cRel^−/−, Relb^−/−cRel^−/−, and IκBα^−/−IκBε^-/cRel^−/−TNF^−/− mice. The gating strategy used in the analysis is depicted in Figure 3. Numbers represent percentage of denoted subsets as proportion of total B cells. Representative FACS results of 3 experiments shown.