Figure 2
Endothelialized microfluidics. (A) Aggregates form in endothelialized microfluidics infused with 50 mM FeCl3 and whole blood, washed RBCs, washed platelets, PPP, and PRP. Endothelial cells (blue: Hoescht), platelets (green: CD41), fibrinogen (red). (B) Aggregate formation over the length of the microchannel when whole blood is exposed to 50 mM FeCl3. (C) Endothelial cell viability is concentration dependent with increasing cell death at higher concentrations; left to right: 5 mM, 50 mM, 500 mM, 1 M (green/live: calcien, red/dead: propidium iodide). All scale bars represent 50 μm; N = 5.
