Normal hematopoiesis after Vav-Cre–mediated complete inhibition of Wnt secretion from hematopoietic cells. (A) Complete excision of Porcn in blood samples of Vav-Cre/Porcn^Del mice was assessed by PCR of gDNA (Del, excised Porcn allele; FL, floxed allele; WT, wild-type allele). Each lane represents an individual mouse from 8 independent experiments. (B) Expression of Wnt target genes Axin2, c-Myc, and Cyclin D1 was not downregulated in Porcn inactivated BM samples. No expression of Porcn was detected in BM samples of Vav-Cre/Porcn^Del mice (n = 7 per group [3 independent experiments, *P < .05, NS P > .05, Mann-Whitney test]). (C) Similar myeloid progenitor cell numbers were observed in Vav-Cre/Porcn^WT and Porcn^Del mice. Graphs represent the CMP, MEP, and GMP cell numbers from 1 leg (n = 6 per group, 3 independent experiments, NS P > .05 Mann-Whitney test). (D) The CLP cell numbers (1 leg) were not significantly different in Vav-Cre/Porcn^WT and Porcn^Del mice (n = 6 mice per group, 3 independent experiments, P > .05 Mann-Whitney test). (E) The LT-HSC, ST-HSC, HPC numbers (1 leg) were not significantly different in 1-year-old Vav-Cre/Porcn^WT and Porcn^Del mice (n = 6 mice per group, 2 independent experiments, P > .05 Mann-Whitney test). (F) The LT-HSC, ST-HSC, HPC numbers (1 leg) were not significantly different in 6- to 8-week-old Vav-Cre/Porcn^WT and Porcn^Del mice (n = 4 mice per group, P > .05 Mann-Whitney test).