Figure 1
Figure 1. EM analysis of FVIII in complex with dimeric and monomeric D′D3. (A) Representative raw image of dimeric FVIII-D′D3 in negative stain. Some of the complexes are circled. The scale bar represents 50 nm. (B) Selected class averages of dimeric FVIII-D′D3 complex illustrating that the angle between the 2 FVIII molecules varies greatly. White arrows indicate the density representing the dimeric D′D3 domain. The side length of individual panels is 75.1 nm. (C) Representative raw image of monomeric FVIII-D′D3 in negative stain. The scale bar represents 50 nm. (D) Selected class averages of monomeric FVIII-D′D3 complex illustrating that structural heterogeneity persists in this complex. White arrows indicate the density representing the monomeric D′D3 domain. The side length of individual panels is 33.4 nm.

EM analysis of FVIII in complex with dimeric and monomeric D′D3. (A) Representative raw image of dimeric FVIII-D′D3 in negative stain. Some of the complexes are circled. The scale bar represents 50 nm. (B) Selected class averages of dimeric FVIII-D′D3 complex illustrating that the angle between the 2 FVIII molecules varies greatly. White arrows indicate the density representing the dimeric D′D3 domain. The side length of individual panels is 75.1 nm. (C) Representative raw image of monomeric FVIII-D′D3 in negative stain. The scale bar represents 50 nm. (D) Selected class averages of monomeric FVIII-D′D3 complex illustrating that structural heterogeneity persists in this complex. White arrows indicate the density representing the monomeric D′D3 domain. The side length of individual panels is 33.4 nm.

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