Rapid hemolysis caused by the IV administration of H₂O is associated with increased leukocyte (WBC) recruitment in C57BL/6 mice. WBC (A) rolling, (B) adhesion, and (C) extravasation in the cremaster microcirculation, determined at 180 minutes after administration of TNF-α (0.5 µg IP) or vehicle or 15 minutes after H₂O (150 µL IV) or saline. ***P < .001 compared with vehicle; ##P < .01 and ###P < .001 compared with saline; n = 28 to 53 venules from 3 to 6 mice. (D) Representative images of C57BL/6 mice venules after treatments. White stars represent adherent WBCs; white arrow indicates the direction of blood flow. Scale bar, 15 µm.