Figure 1
Figure 1. Tmod3 is expressed in platelets and MKs. (A) Western blotting of Tmod expression in human and mouse platelets. (B) Western blotting of Tmods in MKs cultured from mouse fetal liver (E13.5); 10 ng purified recombinant Tmod1 and Tmod3 proteins were used as controls (A-B). (C) LacZ staining of sections from Tmod3+/+ (left) and Tmod3+/− (right) E14.5 embryos. Embryos were dissected and stained with X-gal and H&E. Yellow arrows indicate large MKs with bright blue staining. Bar, 40 µm. Images were acquired with a ×20 objective (N.A. 0.5) using a Zeiss Axioskop microscope and a Zeiss AxioCam ICc3 color camera. (D) Immunofluorescence staining for β-galactosidase (green) and CD41 (red) in cryosections of Tmod3+/+ and Tmod3+/− E14.5 embryos. Bar, 20 µm. Images were acquired using a Zeiss LSM 780 confocal laser scanning fluorescence microscope with a ×60 oil-immersion objective (N.A. 1.4).

Tmod3 is expressed in platelets and MKs. (A) Western blotting of Tmod expression in human and mouse platelets. (B) Western blotting of Tmods in MKs cultured from mouse fetal liver (E13.5); 10 ng purified recombinant Tmod1 and Tmod3 proteins were used as controls (A-B). (C) LacZ staining of sections from Tmod3+/+ (left) and Tmod3+/− (right) E14.5 embryos. Embryos were dissected and stained with X-gal and H&E. Yellow arrows indicate large MKs with bright blue staining. Bar, 40 µm. Images were acquired with a ×20 objective (N.A. 0.5) using a Zeiss Axioskop microscope and a Zeiss AxioCam ICc3 color camera. (D) Immunofluorescence staining for β-galactosidase (green) and CD41 (red) in cryosections of Tmod3+/+ and Tmod3+/− E14.5 embryos. Bar, 20 µm. Images were acquired using a Zeiss LSM 780 confocal laser scanning fluorescence microscope with a ×60 oil-immersion objective (N.A. 1.4).

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