Figure 2
![Figure 2. αDR3-treated T cells are less proliferative and maintain higher Treg frequency. T cells from αDR3 or isotype mAb–treated C57BL/6 mice were plated at 1:1 ratio to γ-irradiated Balb/c splenocytes in an MLR. (A) [3H] Thymidine incorporation of allogeneic responder cells (**P < .01, ***P < .001, ****P < .0001). (B) T cells were stained for FoxP3 before and 96 hours after MLR to assess Tregs (%) of CD4+ T cells (***P < .001). (C-E) Frequencies of TNFα (C), granzyme B (D), and T-bet (E) in CD8+ T cells are shown (*P < .05, ***P < .001). The results of more than 2 independent experiments are shown (N = 4, in total).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/126/4/10.1182_blood-2015-04-637587/4/546f2.jpeg?Expires=1724971114&Signature=kVBGaFpovOKpWQVpuYdf0lMvGfhCCaVeroqGA-EkR8hEiMgHm~jviiDuweWkxDozs~8i6VbWKQ48ejfR0qAy0FWpH7mtlubnt5RyrqmKghHwvt6NQt27NL9aKEi47~gbq5Tsvxhjel~R39HYCw-kPwYtQwnx6peCedtryUY6~jpU4O1hmkvC4cProI~Xv4U6hE0EFys4TeXkONj073ZZVSYp7HxUG3ieWBO-F1HChsA3Q~2YZ3kkNF4betW2bE6eQtnQWYp3uBWTUF9qiCh9bWxQR2SSBfni5zis5lBtU5ngglrUnwraU4~WTnxg8Vdd~kc~-J-3y2liz5wLYQ4UYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
αDR3-treated T cells are less proliferative and maintain higher Treg frequency. T cells from αDR3 or isotype mAb–treated C57BL/6 mice were plated at 1:1 ratio to γ-irradiated Balb/c splenocytes in an MLR. (A) [3H] Thymidine incorporation of allogeneic responder cells (**P < .01, ***P < .001, ****P < .0001). (B) T cells were stained for FoxP3 before and 96 hours after MLR to assess Tregs (%) of CD4+ T cells (***P < .001). (C-E) Frequencies of TNFα (C), granzyme B (D), and T-bet (E) in CD8+ T cells are shown (*P < .05, ***P < .001). The results of more than 2 independent experiments are shown (N = 4, in total).
