Tumor-infiltrating naive CB T cells rapidly gain effector functions such as IFN-γ, TNF-α and perforin. (A-C) Representative flow cytometry dot plots and cumulative bar plots (mean and standard error of mean) of percentages of CB and PB CD8⁺ TILs secreting IFN-γ, TNF-α, and perforin expression. Although no statistically significant differences were observed in the percentage of IFN-γ–secreting CD8⁺ TILs in the 2 groups, a significantly higher percentage of CD8⁺ TILs in the CB group secreted TNF-α. Perforin expression was also significantly higher in the CB CD8⁺ TILs than PB CD8⁺ TILs and is depicted as median fluorescence intensity and percentage of CD8⁺ TILs.