Reduction in reticulocyte number and increased lifespan in RN-1–treated SCD mice. (A) The percentage of reticulocytes was measured by flow cytometry after thiazole orange staining of whole blood. The number shown above the horizontal bar in each box represents the fractional percentage of reticulocytes among the total red blood cells in each sample. (B) Statistical analysis of the percentage of reticulocytes in all samples from untreated and RN-1–treated SCD mice. Data are presented as mean ± SD (n = 4-6 mice per group) (*P < .05; **P < .01 vs untreated SCD mice). (C) Lifespan of erythroid cells in untreated or RN-1–treated SCD mice (data are depicted as the mean ± SD, n = 3 mice per group).