Knockdown of Rpl11 in zebrafish embryos model the erythroid and developmental defect in DBA. Rpl11 in embryos were targeted using a translational start codon morpholino. (A) Western blot against Rpl11 in 24 hpf embryos injected with various doses of morpholino. (B) Transcript quantification of p53, mdm2, and p21 at 24 hpf. (C-D) Morphologic features of morphants at 24 hpf (C) and 48 hpf (D). Arrowheads point to the defective head development and shorted tail extension at 24 hpf. At 48 hpf, a smaller head, edema, and defective yolk extension were observed (arrowhead) in morphants. (E) o-Dianisidine staining of zebrafish embryos at 48 hpf. (F) Flk:dsred transgenic zebrafish at 48 hpf. (G) Mpo:EGFP transgenic zebrafish at 48 hpf.