Figure 3
![GO-203 increases hydrogen peroxide (H2O2) levels in CTCL cells, which is abrogated by addition of N acetyl cysteine (NAC). (A-D) HuT-78 and H9 cells were left untreated and treated with 5 μM GO-203 or CP-2 each day for 3 days. (C and D) The GO-203-treated cells were also incubated in the presence of 5 mM NAC for the last 2 days. Cells were incubated with c-H2DCFDA for 30 minutes. Fluorescence of oxidized DCF was measured by flow cytometry (left). The results are expressed as the relative hydrogen peroxide level (mean ± standard deviation [SD] of 3 determinations) compared with that obtained for control cells (right).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/126/3/10.1182_blood-2015-02-628149/4/354f3.jpeg?Expires=1721843896&Signature=YRn7ieWgA3n50P971vJ79ga7VfKVOivyqIbA8eYOxAttHGfFC3~iLJyTmwH2dvw4tpGRBh5M06wbl~tolZUDWrZY-Jyr6zjJCO4M7Xhw7vg-1dzBUBx8ZkE38umwUqKDI0R2YZZz6qmZHfldMbNv0c7rrptj7vyRG8x-TLluxpmYLsd9OzOJb2aYGpK2IUCGeMDHjQVTSFavSkgl0HqqYKySoPwvRSCmJQP729hN-TwxQbPZEnQCyi95nMhrmGBx1Dm1dhTKCGutgjIgA7hNW0OOyNnI0fDSbbgIDxBCtxyUt23700PcyWBf4NEEGEEx1v~zKeDOu-uoPfHScwbG-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
GO-203 increases hydrogen peroxide (H2O2) levels in CTCL cells, which is abrogated by addition of N acetyl cysteine (NAC). (A-D) HuT-78 and H9 cells were left untreated and treated with 5 μM GO-203 or CP-2 each day for 3 days. (C and D) The GO-203-treated cells were also incubated in the presence of 5 mM NAC for the last 2 days. Cells were incubated with c-H2DCFDA for 30 minutes. Fluorescence of oxidized DCF was measured by flow cytometry (left). The results are expressed as the relative hydrogen peroxide level (mean ± standard deviation [SD] of 3 determinations) compared with that obtained for control cells (right).
