Selective expression of MUC1 in CTCL cells. (A) Sézary (HuT-78, H9, and SeAx) and mycosis (Myla, HuT-102) cell lines were analyzed for MUC1-C messenger RNA expression by RT-PCR (Michigan Cancer Foundation-7 breast adenocarcinoma cell line [MCF-7] used as positive control, GAPDH as loading control) in comparison with normal T cells from healthy individual and diffuse large B-cell lymphoma (DLBCL; Toledo, OCI-LY3, Val, RCK-8, and SUDHL-2), Burkitt lymphoma (Ramos and Daudi), and mantle cell lymphoma (JVM-2) cell lines. (B) Sézary, mycosis, and normal T cells from healthy individuals; additional DLBCL (OCI-LY7 and SUDHL-5), Burkitt lymphoma, and mantle cell lymphoma cells were also analyzed for the oncogenic subunit MUC1-C by immunoblotting (Chronic myeloid leukemia [CML] and MCF-7 used as positive controls). (C-D) Sézary and mycosis cell lines were further analyzed for MUC1 expression by flow cytometry (C) in comparison with additional DLBCL cell lines (SUDHL-8, K422, OCI-LY3, and OCI-LY8) (D). The difference in the mean fluorescent intensity between unstained, isotype control, and MUC1-stained cells are highlighted in each histogram in the top.