Figure 5
Resistance to DEX and functional activity of TALEN-modified CD8+ T cells in a xeno-GVHD model. (A) Hair loss (top row) and skin infiltration with human CD45+ cells counterstained with hematoxylin (middle row; inset shown in bottom row) from irradiated NSG mice injected with 1 × 106 CD8+wt or CD8+GR-Ex2 T cells and treated daily or not with intraperitoneally injected DEX at 15 mg/kg for 21 days. (B) Absolute number of human GZB-expressing (GZB+) CD8+wt or CD8+GR-Ex2 T cells in the spleens of previously described NSG mice. (C) Western blot analysis of GR expression from human-selected cells in pooled spleens ex vivo and at day 3 after transfection with TALENs. Data are representative means from 2 independent experiments with 5 mice per group; statistical analysis was performed by 1-way ANOVA (***P < .001) with Bonferroni multiple comparisons.

Resistance to DEX and functional activity of TALEN-modified CD8+ T cells in a xeno-GVHD model. (A) Hair loss (top row) and skin infiltration with human CD45+ cells counterstained with hematoxylin (middle row; inset shown in bottom row) from irradiated NSG mice injected with 1 × 106 CD8+wt or CD8+GR-Ex2 T cells and treated daily or not with intraperitoneally injected DEX at 15 mg/kg for 21 days. (B) Absolute number of human GZB-expressing (GZB+) CD8+wt or CD8+GR-Ex2 T cells in the spleens of previously described NSG mice. (C) Western blot analysis of GR expression from human-selected cells in pooled spleens ex vivo and at day 3 after transfection with TALENs. Data are representative means from 2 independent experiments with 5 mice per group; statistical analysis was performed by 1-way ANOVA (***P < .001) with Bonferroni multiple comparisons.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal