Red cell characteristics of RBC10 mice. (A) Peripheral blood smears from 7-week-old wild-type (+/+), RBC10 heterozygous (+/m), and homozygous (m/m) mice. (B) Osmotic fragility shows significantly reduced sensitivity of RBC10 mutant red cells. Plots are representative of 2 independent experiments using 3 animals of each genotype. (C) Red cell densities of wild-type, RBC10 heterozygous, and RBC homozygous mice determined by centrifugation through Percoll mixture of defined density. Student t test: wild type compared with RBC10 heterozygous: *P < .05, **P < .01, ***P < .001. RBC10 homozygous compared with heterozygous: ^#P < .05.