Significant association with grade III-IV aGVHD detected in 12p12.1. Manhattan plot (A) and QQ plot (B) of GWAS analysis. A log-rank trend test statistic was calculated to compare the cumulative rates of grade III-IV aGVHD, in which analyses were performed based on the number of allele mismatches between donor and recipient. Under the assumption that the minor H antigen was presented by HLA-DQB1*06:01, association was tested within the subgroup of transplants sharing HLA-DQB1*06:01. In the QQ plot, 95% CIs are depicted in gray. (C) Cluster plot of rs17473423 showing the highest significance. Specimens genotyped as AA, AG, and GG are shown in blue, green, and red, respectively. (D) Regional association plots are depicted in which unobserved SNPs were imputed using the data from the 1000 Genomes Project. Genetic recombination rates estimated using genotyping data from the 1000 Genomes Project are depicted by a light blue line; r2 values between SNPs and the most significantly associated SNP (rs11047903; “Top”) are color-coded.
