SNPs around the HLA-DPB1 locus showed strong association with the development of grade II-IV aGVHD. Manhattan plot (A) and QQ plot (B) of GWAS analysis, in which the log-rank test statistic was calculated for each SNP according to the number of mismatching alleles in the GVH direction between the donor and patient. In the QQ plot, 95% CIs are depicted in gray. (C) Unobserved SNPs were imputed using the data from the 1000 Genomes Project. The P values from the log-rank test for each SNP around the HLA-DPB1 locus were plotted as circles, the color of which indicates the r2 value between each SNP and the SNP showing the minimum P value (rs114477774; “Top”). In the diagram, genetic recombination rates are overlaid with a light blue line. (D) Cluster plot for rs6937034. Specimens genotyped as AA, AG, and GG are shown in red, green, and blue, respectively. CI, confidence interval; QQ, quantile-quantile.
