Decreased self-renewal ability of HSCs in the ATF4^−/− FL. (A) Representative flow cytometric analysis of HSCs in the FL at E14.5. FSC-A, forward-scattering angle. (B-C) Frequency and number of SLAM cells in the FL at E14.5 (n = 4 for each genotype). (D-E) Donor chimerism and RU of 30 SLAM cells at different time points after transplantation (n = 12 for each group). (F) Donor chimerism of secondary transplantation using 1 × 10⁶ donor-derived BMNCs (CD45.2) and 1 × 10⁵ competitor BMNCs (CD45.1). (G) Number of RUs in 1 × 10⁶ primary donor cells at 16 weeks after secondary transplantation (n = 12). Student t test *P < .05, **P < .01, and ***P < .001.