Use of slan for dissection of CD16-positive monocyte subsets. Whole blood from a healthy control (ID 2059) was stained with monoclonal antibodies to CD14, CD16, HLA-DR, and slan and analyzed in 4-color flow cytometry. Monocyte subsets defined in the CD14 CD16 dot plot (top) are classical (417.6 cells per microliter), intermediate (16.8 cells per microliter), and nonclassical monocytes (39.3 cells per microliter). Separation based on slan reveals slan-positive nonclassical monocytes (26.3 cells per microliter) (bottom right) and slan-negative intermediate monocytes (31.2 cells per microliter) (bottom left).