LY2510924 induces myeloid differentiation of AML cells in organs of OCI-AML3 xenograft model. (A) In the OCI-AML3/Luc/mCherry xenograft model, BM and liver samples of representative control and LY2510924-treated mice were fixed and sectioned for immunohistochemical analysis with antihuman CD11c antibodies to investigate myeloid differentiation of leukemic cells. Low magnification of BM sections shows that leukemic cells almost completely efface the BM medullary space in the control mouse whereas clusters of large pale leukemic cells are intermixed with small darker normal hematopoiesis in the LY2510924-treated mouse. Low magnification of liver sections also demonstrates that the periportal sheets of neoplastic cells are significantly larger in the control compared with the LY2510924-treated mouse. At high magnification, the leukemic cells demonstrate similar morphology in BM and liver in both control and treated mice. However, anti-CD11c antibody demonstrates more differentiated cells in BM and liver of a treated mouse compared with BM and liver of a control mouse. (B) The CD11c expression was assessed by manually counting positive and negative signal in 200 leukemic cells, which were discriminated from mouse cells based on size and nuclear chromatin characteristics; the bar graph demonstrates the difference between groups. H&E, hematoxylin and eosin.