Suz12 is required for fetal hematopoiesis. (A) Representative plots of flow cytometric analyses of hematopoietic stem and progenitor cells in fetal livers of Suz12^fl/+, Suz12^fl/Δ, Suz12^fl/+/VavCre^T, and Suz12^fl/Δ/VavCre^T E13.5 embryos. Numbers from gated populations represent the proportions of total viable fetal liver cells of the indicated populations: lineage⁻ Sca-1⁺ c-Kit⁺ (LSK) cells (top panel), LSK CD150⁺ CD48⁻ cells (middle panel) and myeloid progenitors (CMP, MEP, and GMP; lower panel) are shown. The absolute numbers of hematopoietic stem and progenitor cells in E13.5 fetal livers are summarized in the adjacent bar charts. (B) Representative flow cytometric plots are shown of erythroid differentiation in fetal livers of Suz12^fl/+, Suz12^fl/Δ, Suz12^fl/+/VavCre^T, and Suz12^fl/Δ/VavCre^T E13.5 embryos. The absolute cell numbers in each stage of erythroid differentiation are summarized in the adjacent bar chart. Data represent mean ± standard deviation from n = 5-14 fetal livers per genotype. One-way analysis of variance followed by Tukey’s post-hoc test was performed to compare differences between genotypes. *P < .05; **P < .01.